Table 3.

KEGG pathway analysis of the differentially methylated genes in neural tissues from NTD cases as compared to controls^a.

Pathway_ID	Term	P-value	Gene symbol	Fold enrichment	Bonferroni	Benjamini
hsa04810	Regulation of actin cytoskeleton	4.29E-03	FGF19, FGFR2, FGFR4, APC2, INS-IGF2, BCAR1, RDX, BDKRB2, PFN3, KRAS, PAK2, TIAM2, TIAM1, PAK1, FGF1, PIK3R1, ARHGEF7, CHRM3, CHRM2, RRAS2, CHRM1, ITGA7, CYFIP2, MYLK, MYH10	1.80	0.53	0.12
hsa04310	Wnt signaling pathway	1.43E-04	PRKCA, TBL1XR1, CTBP1, CTBP2, APC2, PPP2R5C, VANGL2, MMP7, TP53, SMAD3, FZD3, MAPK10, DAAM2, CCND1, SFRP2, PPP2CA, FRAT1, SIAH1, CAMK2A, NFATC1, WNT8B	2.37	0.02	0.02
hsa04530	Tight junction	1.86E-04	PRKCA, PRKCZ, CLDN19, VAPA, CLDN6, MYH2, MPP5, MYH7, CLDN20, CDK4, CTNNA1, CLDN14, CTNNA2, EPB41L2, KRAS, PPP2CA, RRAS2, PPP2R2B, PPP2R2C, TJP2, PTENP1, MYH10	2.45	0.03	0.02
hsa04360	Axon guidance	2.43E-02	PLXNB1, PLXNA2, PLXNB2, DPYSL5, KRAS, PAK2, FYN, RGS3, SEMA3B, PAK1, SEMA4D, NFATC1	1.85	0.99	0.38
hsa04960	Aldosterone-regulated sodium reabsorption	1.24E-03	PRKCA, SGK1, KRAS, INS-IGF2, HSD11B1, NR3C2, NEDD4L, SCNN1A, PIK3R1, KCNJ1	3.64	0.19	0.07
hsa00980	Metabolism of xenobiotics by cytochrome P450	4.47E-02	CYP1B1, CYP2C19, ADH4, ALDH1A3, EPHX1, MGST1	2.24	1.00	0.51
hsa00534	Heparan sulfate biosynthesis	2.66E-02	EXTL3, EXT1, HS3ST3B1, GLCE	3.44	0.99	0.37

^aKEGG, Kyoto Encyclopedia of Genes and Genomes.

P-value was obtained with modified Fishers exact statistic and adjusted for multiple testing with Bonferroni and Benjamini methods