Figure 6.

SIX2, a developmental TF gene, exhibits three hypermethylated DMRs in SkM and aorta that correlate with specific gene expression in these tissues. (a) SIX2, the LINC01121 ncRNA gene (dashed line) and RNA-seq for the minus-strand of cell cultures and non-strand-specific RNA-seq for tissues (chr2:45,224,940–45,243,926). (b) Chromatin state segmentation; dashed box, the SIX2 gene body for reference. (c) Significant hyper- or hypo-methylated CpG sites from RRBS profiles.²³ (d) and (e) Bisulfite-seq and TAB-seq. Dashed boxes, Mb/Mt- and SkM-hypermethylated region; purple box, subregion in osteoblasts, SkM, and aorta lacking DNA hypermethylation observed in Mb and Mt. Arrowhead, the CCGG site analyzed for by Epimark assay and shown to have the highest 5hmC in SkM but only with an average of 7% of all C as 5hmC (Fig. 2a).