Fig 5. Effect of acute serelaxin treatment on renal blood flow and tissue oxygenation in CCl₄ cirrhotic rats.

Renal blood flow (RBF, A) and mean arterial pressure (MAP, B) responses to acute i.v. serelaxin (4 μg) or vehicle in 16-wk CCl₄ rats (n = 5–7). Measurement of velocity time integral (C) and renal resistive index (D) following acute i.v. serelaxin (4 μg) or vehicle (n = 6–8). Deoxygenated hemoglobin levels (R2*) in renal medulla in 8-wk (E) and 16-wk (F) CCl₄ rats at baseline, 30 min, and 60 min following acute i.v. serelaxin (4 μg) or vehicle (n = 5–8). Data presented as mean ± standard error of the mean, analyzed by two-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001; NS, not significant) with post hoc Bonferroni correction to compare individual CCl₄ time points with respective vehicle controls (^#p < 0.05; ^##p < 0.01; ^###p < 0.001).