Figure 1.

Increased lactate production and decreased complex I activity in AIF-deficient cells. (A) Number of divisions per day of AIF^−/y as compared to AIF^+/y ES cells. Values are given as mean±s.d. (n=12). (B) Acidification of media by AIF^−/y and AIF^+/y cells, as determined in nonconfluent cultures (2.5 × 10⁵ cells/ml at day 0). (C) Lactate production by AIF^−/y and AIF^+/y ES cells, as evaluated at different densities (mean±s.e.m., n=3). Asterisks indicate significant (P<0.01, unpaired Student's t-test) differences between AIF^−/y and AIF^+/y cells.