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. 2017 Feb 28;12(2):e0172913. doi: 10.1371/journal.pone.0172913

Fig 2. Construction of conjugative interference plasmids.

Fig 2

pJIMK46 (A, B) was constructed from pJIBE401 by replacing 28.5 kb including the MRR with tetA and then part of the pemK toxin gene with fosA3. pJIMK56 (C, D) was constructed from pJIE512b by replacing the blaCMY-2 gene and flanking IS with fosA3 and then part of the pndA toxin gene with tetA. Numbers indicate the positions of the amplified regions in GenBank accession nos. JX101693.1 (pEl1573) or HG970648.1 (pJIE512b). Blue and red arrows indicate overlapping primers, black arrows indicate other primers.