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. 2017 Feb 28;12(2):e0172913. doi: 10.1371/journal.pone.0172913

Table 3. Protocol for mouse experiments.

Day(s) Group 1 Group 2 Group 3 Group 5 Group 4 Group 6
Controls Antibiotics Antibiotics+resistance plasmid Antibiotics+resistance+interference plasmid
-6-0 acclimatization—unrestricted normal diet and water
1–3 run-in of gelatine food protocol (6 h fast, gelatine food 3 h, unrestricted normal diet and water)
4–6 gelatine gelatine+CTX gelatine+CTX+(J53Azir+pEl1573)~2x106 cfu/cage/day gelatine+CTX+ (UB5201Rf+pJIE512b)~2x106 cfu/cage/day gelatine+CTX+(J53Azir+pEl1573)~2x106 cfu/cage/day gelatine+CTX+ (UB5201Rf+pJIE512b)~2x106 cfu/cage/day
7 unrestricted normal diet and water
8–10 gelatine gelatine+TET gelatine gelatine gelatine+TET+(UB5201Rf+pJIMK46)~6x107 cfu/cage/day gelatine+TET+(J53Azir+pJIMK56)~7x107 cfu/cage/day
11 unrestricted normal diet and water
12 move to clean cages
13–22 unrestricted normal diet and water
23–24 unrestricted normal diet and water CTX in water
28 end of experiment