Table 1. Standard primers used for gene cloning.
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|
GPI | GGCACATACATGGTGTGAGG | TCCTAAATGCCGGAACATAA |
PMI | TGAAGCACCACAATCAAAGG | TGCTTGCACTAAAGCCAAGA |
PMM | CCATTTCGGAGCTCTCATTC | CCGAAACCAAAAAGTTGGAA |
GMP | GGAAAGCAAAGGGCATATCA | CAGCCTGAATCCAGAGAAGC |
GME | GCCTTGTTTCCTTTGCTCTG | CTGAGCAATGCTTCAGATGG |
GGP1 | TCCCTCCGATCTTACCTTCC | TTGCTGAGCGTCTATTTCCA |
GGP2 | CATCACGGCTATACACGGAG | ATAAACCCAAATGGCAAACT |
GPP | CCGCATTTCCTTCTCTCATC | AGCTTCGAGCAAGATTACCG |
GalDH | ATATTTTTCGCTTCACTTCC | ATTTTATTGATTCATCTCCA |
GalLDH | AAAGAGGGAAGGAGGAGCAG | TGAAGCCAAAATGCATTCAA |
APX1 | TCCTTCCCTCCAAACAACAC | CACACATAGGCAACCAATGC |
APX2 | CTACCCAAACAGAGGGTGGA | AGCCATCGCTGCCTTATTTA |
APX3 | AATGGCGTTCTCCTTCCTTT | AGCTATCCGTGGGTCAAACA |
AO1 | TGTCTCTTTCTGTAACCTTC | TTCTTTCTTTTTCCTCTCTC |
AO2 | TTTTAACTTGTTGTAGTTTG | ATAGTTGAATGGTATGAGAT |
DHAR1 | CTGGCACTCTTCCTCACTTC | CTTCCTTTTTTTGGCTATCT |
DHAR2 | ACACCGACCAATGCGTTAAT | TCCACATCTGACATGCACAC |
GR1 | TCATGGCCACCTCTCTCTCT | CATGCTCTGCAGAACCTCAA |
GR2 | GCTTCGCCATCTTCAACTTC | CGTCCTATTGCTTGCATCCT |
MDHAR | GCCAGCCACTGTCCTTAAAC | TGCAAAGGAGACAGCTTCAA |