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. 2004 Nov 4;23(23):4606–4614. doi: 10.1038/sj.emboj.7600465

Figure 1.

Figure 1

Reconstitution of the mouse mitochondrial transcription system in vitro. (A) SDS–PAGE gel stained with Coomassie blue showing the recombinant mouse proteins used for in vitro transcription reaction. Whereas mTFAM is expressed on its own, mPOLRMT is co-expressed and co-purified with mTFB2M. (B) Transcription from mLSP only occurs when mTFAM (2.5 pmol), mPOLRMT (500 fmol) and mTFB2M (500 fmol) are present simultaneously. (C) The human and mouse transcription machinery cannot initiate transcription from heterologous promoters. Linear templates (85 fmol) containing the mouse or hLSP were used for in vitro run-off transcription assays. The reactions were performed with the following pure recombinant proteins: human or mouse POLRMT (500 fmol), human or mouse TFB2M (500 fmol) and human or mouse TFAM (2.5 pmol).