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. 2004 Nov 4;23(23):4606–4614. doi: 10.1038/sj.emboj.7600465

Figure 5.

Figure 5

(A) Mouse TFAM can efficiently support transcription on hLSP. Template containing the LSP (85 fmol) of human mtDNA was used for in vitro run-off transcription assays. The reactions were performed with the following pure recombinant proteins: hPOLRMT (300 fmol), hTFB2M (300 fmol) and hTFAM or mTFAM in increasing amounts (0, 0.025, 0.1, 0.25, 1.0, 2.5, 10 pmol). (B) Mouse POLRMT cannot initiate transcription at hLSP. In vitro transcription from hLSP was performed with the proteins indicated (500 fmol) in the presence of (2.5 pmol) of hTFAM.