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A. Expression of genes associated with browning (Ucp1, Fndc5, Cidea, Adrb3) from EWAT, IWAT, and BAT (normalized to HFHS-fed EWAT). B. Representative images of UCP1 immunostaining from EWAT, IWAT, and BAT, scale bar = 300 μm. Percent total UCP1 stained area for EWAT (left), IWAT (middle), and BAT (right) shown below. Data are presented as mean ± SEM, n = 5-15/group. *P<0.05 from HFHS control, **P<0.05 from CR.
