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In vitro phenotypes of editing defective PheRS. (A) Hydrolysis of Tyr-tRNAPhe (1 μM) by PheRS (2 nM). (B) Aminoacylation of tRNAPhe (2.7 μM) with Tyr (30 μM) by PheRS (250 nM). Wild-type PheRS (▴), αA294G PheRS (▪), αA294G-βH265A (▵), αA294G-βE334A (•), αA294G-βA356W (▾), no enzyme (◊).
