OCTs Spectral / Sw. source |
OCM (Scanning) |
OCM (Coh. illumination) |
OCM (Incoh. illumination) |
|
---|---|---|---|---|
Axial: Transverse: Resolution |
3 to 10 μm 10 μm |
3 to 8 μm 0.5 to 2 μm |
3 to 8 μm 0.5 to 2 μm |
0.5 to 8 μm 0.5 to 2 μm |
Speed (Pixels/s) |
100 Gpixel/s | 1-10 Gpixels/s >100 Gpixels/s with Spectral or Sw. source. | 10-50 Gpixels/s 1 processed image | 10-50 Gpixels/s 1 processed image |
Sources | SLD/ Lasers (fs) |
SLD/ Lasers (fs) |
SLD/ Lasers (fs) |
Thermal Sources LEDs |
Typ. Power (sample) Sensitivity |
10-100 mW 100/110 dB |
10-100mW 100/110 dB | 1 mW | 0.5-2 mW 80-90 dB |
Aberrations Corrections | With Adaptive Optics | With Adaptive Optics | With Adaptive Optics | No loss of resolution (only signal reduction) |
Sensitivity to movements | Correction possible | Correction possible | Correction unlikely | Correction unlikely |
Post-processing Depth of field and aberration correction |
Synthetic aperture, Aberrations correction | Depth of field and aberration correction achieved | Numerically possible | Difficult |
Main Drawbacks | Low resolution | Price of fs lasers | Crosstalks | Lower sensitivity: camera saturation. Sensitive to movements |
Main Advantages | Broadly used Easy to implement | S/N. Coupling with non-linear microscopy | Possible wavefront shaping | Cheap, High resolution. Sub-cellular metabolism |