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. 2017 Jan 6;8(2):622–639. doi: 10.1364/BOE.8.000622
OCTs
Spectral /
Sw. source
OCM
(Scanning)
OCM
(Coh. illumination)
OCM
(Incoh.
illumination)
Axial:
Transverse:
Resolution
3 to 10 μm
10 μm
3 to 8 μm
0.5 to 2 μm
3 to 8 μm
0.5 to 2 μm
0.5 to 8 μm
0.5 to 2 μm
Speed
(Pixels/s)
100 Gpixel/s 1-10 Gpixels/s >100 Gpixels/s with Spectral or Sw. source. 10-50 Gpixels/s 1 processed image 10-50 Gpixels/s
1 processed image
Sources SLD/
Lasers (fs)
SLD/
Lasers (fs)
SLD/
Lasers (fs)
Thermal Sources LEDs
Typ. Power (sample)
Sensitivity
10-100 mW
100/110 dB
10-100mW 100/110 dB 1 mW 0.5-2 mW
80-90 dB
Aberrations Corrections With Adaptive Optics With Adaptive Optics With Adaptive Optics No loss of resolution (only signal reduction)
Sensitivity to movements Correction possible Correction possible Correction unlikely Correction unlikely
Post-processing
Depth of field and aberration correction
Synthetic aperture, Aberrations correction Depth of field and aberration correction achieved Numerically possible Difficult
Main Drawbacks Low resolution Price of fs lasers Crosstalks Lower sensitivity: camera saturation. Sensitive to movements
Main Advantages Broadly used Easy to implement S/N. Coupling with non-linear microscopy Possible wavefront shaping Cheap, High resolution. Sub-cellular metabolism