Figure 6. Itgα6−/− CD cells show adhesion, migration and adhesion strength defects on LM-332.
Adhesion (A), migration (B), proliferation (C), and replating assay (D) on LM-322 (1 μg/ml) were evaluated as described in the Methods and in Fig. 5. Shown are mean measurements ±SEM of 4–6 independent experiments; *p<0.01between Itgα6−/− and Itgα6f/f CD cells. (E) The shear stress for 50% detachment (τ50), which represents the mean cell adhesion strength, was determined for Itgα6−/− and Itgα6f/f CD cells on LM-332 and fibronectin (which is not a ligand of a6 containing integrin) coated cell culture plates. Shown are the averaged τ50 values of 3 independent experiments; *p<0.01between Itgα6−/− and Itgα6f/f CD cells.