Table 2.

Fidelity of LOOPER with a heterofunctionalized library^[a]

Entry	Pentanucleotide	R group[b]	Enrich[c]	Bias[d]	Fidelity[e]
1	5’P-A*NNNN	R1-R16	0.99	0.41	93.8%
2	5’P-A*NNAA		0.71	0.75	93.9%
3	5’P-A*NNCA		1.01	1.03	93.8%
4	5’P-A*NNGA		1.04	1.07	94.5%
5	5’P-A*NNTA		0.66	0.69	89.3%
6	5’P-A*NNAC		0.91	0.94	87.3%
7	5’P-A*NNCC		1.48	1.49	96.6%
8	5’P-A*NNGC		1.70	1.71	96.6%
9	5’P-A*NNTC		0.78	0.78	91.4%
10	5’P-A*NNAG		1.03	1.08	93.9%
11	5’P-A*NNCG		1.18	1.20	96.2%
12	5’P-A*NNGG		1.46	1.50	97.4%
13	5’P-A*NNTG		1.41	1.46	97.5%
14	5’P-A*NNAT		0.74	0.77	89.2%
15	5’P-A*NNCT		0.69	0.70	93.0%
16	5’P-A*NNGT		1.09	1.12	94.8%
17	5’P-A*NNTT		0.69	0.71	89.5%

^[a]LOOPER and duplex barcoding process performed on 15 pmol of DNA template library. 35 amol of product was subjected to duplex DNA sequencing.

^[b]Functional group derived from carboxylic acid used in coupling to C6 amino dA.

^[c]Enrichment calculated as (freq. polymer/freq. template)

^[d]Codon bias was calculated as σ(freq. polymer/freq. template).

^[e]Calculated for pentanucleotide incorporation.