Figure 5. The mechanisms of ATB2 cells on insulin resistance and the role of LTB4R1 in this process.

(A) GTTs and ITTs of HFD-fed B^null mice treated with T cell–depleting antibodies, macrophage-depleting clodronate, or both. (B) Two weeks after ATB2 cell transfer, GTT and ITT of HFD-fed B^null mice pretreated with clodronate or antibodies. (C) Differentiation of IFN-γ⁺CD4⁺ and IFN-γ⁺CD8⁺ T cells after coculture with WT or Ltb4r1KO ATB2 cells (WT ATB2, Ltb4r1KO ATB2), measured by flow cytometry analysis. (D and E) Levels of the cell surface marker CD69 and gene expression of the proinflammatory cytokines Tnfa, Il6, and Il1b in M1 macrophages after coculture with WT or Ltb4r1KO ATB2 cells. Data are presented as mean ± SEM. n = 8 per group (A and B); n = 6 per group (C–E). *P < 0.05; **P < 0.01, 1-way ANOVA with Bonferroni’s post test.