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. 2017 Jan 30;127(3):857–873. doi: 10.1172/JCI90025

Figure 8. URMC-099 and nanoART parenteral coadministration sustains plasma DTG levels.

Figure 8

Mice were injected i.m. with a single dose (45 mg/kg) of nanoDTG and treated with or without daily i.p. injections of URMC-099 (10 mg/kg). (A) On different days, blood was collected, and the plasma DTG concentration was determined by UPLC-MS/MS. (B) On day 14 (D14) and day 28 (D28), mice were sacrificed and DTG levels quantified in different tissues by UPLC-MS/MS. (C–F) On days 14 and 28, total RNA was isolated from splenic and liver tissues, and real-time qPCR was performed for different genes. Values represent the mean ± SEM. *P ≤ 0.05 and **P ≤ 0.01, by Mann-Whitney U test (n = 6 mice per group). (G and H) On days 14 and 28, splenic and liver tissues were collected, and total tissue lysate was analyzed by Western blotting for different autophagy markers. Each lane is representative of 5 animals from each of the groups. Multiple comparisons were corrected for the FDR using the Benjamini-Hochberg method. Six mice were assessed per group.