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. 2017 Feb 20;127(3):1099–1114. doi: 10.1172/JCI90086

Figure 6. Liver endothelial GATA4 is required for maintenance of discontinuous LSEC differentiation.

Figure 6

(A) Expression of LYVE1, STAB2, and CD31 in the liver of Stab2-Cre Gata4fl/fl embryos at E11.5. IHC (upper panels) and quantification (lower panels) based on IF images of LYVE1, STAB2, and CD31 (n = 4). Scale bars: 100 μm. Western blot of LYVE1 and GAPDH with quantification of densitometric data (n = 5) (right). (B) Transmission electron microscopy (TEM) of the liver of Stab2-Cre Gata4fl/fl embryos at E11.5. Arrows indicate formation of a basement membrane (n = 4). (C) Co-IF of STAB2 and CD31 in the liver of Stab2-Cre Gata4fl/fl (n = 4) (left) and Lyve1-Cre Gata4fl/fl (n = 3) (right) embryos at E11.5. Scale bars: 20 μm (left) and 10 μm (right). (D) IF of CD31 in the fetal liver of Stab2-Cre Gata4fl/fl embryos at E10.5 shows increased expression of CD31 in the endothelium of mutant embryos as an indicator of early hepatic capillarization. Scale bars: 20 μm (upper panels) and 10 μm (lower panels) (n = 2). Student’s t test; ***P < 0.001.