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. 2017 Feb 20;127(3):1099–1114. doi: 10.1172/JCI90086

Figure 8. Endothelial GATA4 regulates VE-cadherin expression and junctional stability in liver sinusoidal and continuous ECs in vivo and in vitro.

Figure 8

(A) IHC of VE-cadherin in the liver of Stab2-Cre Gata4fl/fl embryos at E11.5. Scale bars: 50 μm (left). Co-IF of VE-cadherin and LYVE1 in liver of Lyve1-Cre Gata4fl/fl embryos at E11.5 (n = 3). Scale bar: 20 μm (right). (B) Co-IF of VE-cadherin, TOTO-3, and phalloidin of GATA4-transduced HUVECs (n = 3). Scale bars: 60 μm (left) and 30 μm (right). (C) WB and densitometric quantification of VE-cadherin, α-catenin, and β-catenin of GATA4-transduced HUVECs (n = 4). (D) Co-IP of VE-cadherin and β-catenin of GATA4-transduced HUVECs. WB of lysates is shown as control, WB of precipitates (IP) with anti–VE-cadherin, anti–β-catenin, and the respective IgG controls (n = 4). Student’s t test; **P < 0.01.