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. 2017 Feb 20;127(3):1046–1060. doi: 10.1172/JCI89927

Figure 3. Treg-depleted B6AF1-DEREG mice spontaneously develop autoimmune orchitis and produce Abs to LDH3 but not to ZAN.

Figure 3

(A) Kinetics of autoantibody responses to testicular cell antigens (n = 3–43 per time point). (B) Incidence and level of testicular cell autoantibody at 8 weeks between Treg-depleted DEREG mice (n = 106) and WT mice (n = 22) treated with DT. Serum Ab to (C) rLDH3 (n = 5 to n = 10 per group) and (D) ZAN D3p18 (n = 5 to n = 10 per group) in control and Treg-depleted DEREG mice at 8 weeks. (E) Incidences and severity of seminiferous tubules with abnormal spermatogenesis and sperm loss in epididymis at 8 weeks. (F) Representative histopathology of testis and epididymis between DEREG mice treated with DT (bottom) and PBS (top). Arrow points to a cluster of leukocyte-like cells in the tubule lumen (H&E). Original magnification, ×400 (testis); ×200 (epidymis). Data are pooled from 6–12 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, Mann-Whitney U tests (A–D); Kruskal-Wallis tests with Dunn’s post-tests (E).