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. 2017 Jan 30;127(3):814–829. doi: 10.1172/JCI86154

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Copyright © 2017 Pleines et al.

This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Immunolabeling of TPM4 (red), F-actin (purple), α-tubulin (green), and merge of TPM4/F-actin/α-tubulin and DAPI, in control human proplatelets. Scale bar: 25 μm. (B) Investigation of TPM4 (yellow) and F-actin (red) localization of mouse fetal liver cell–derived megakaryocytes by confocal immunofluorescence microscopy. TPM4 (yellow) localizes to the periphery in mature round WT Tpm4^+/+ megakaryocytes. (C) TPM4 is enriched in proplatelet tips and colocalizes with F-actin (depicted by arrows). Scale bars: 20 μm.