Skip to main content
. 2017 Feb 6;127(3):888–898. doi: 10.1172/JCI88508

Figure 1. MYCN overexpression cooperates with Rb loss to drive retinoblastoma.

Figure 1

(A) Strategy to overexpress MYCN in Rb or Rb/p107-deficient retina. Retina-specific Pax6 α enhancer Cre (Pax6 α Cre) drives the expression of rtTA in the developing retina. The TRE-MYCN allele allowed for DOX-dependent expression of MYCN and luciferase in the retina. Image shows an in vivo assessment of bioluminescence in a tumor-bearing animal. (B) Kaplan-Meier curve shows the time to externally evident retinoblastoma in Rb/p107 (Rb/p107 DKO) compared with Rb/p107/TET-MYCN (Rb/p107 DKO MYCN) mice. The time to tumor presence in the first eye (unilateral curve) and second eye (bilateral curve) is shown. (C) H&E-stained eye from an Rb/p107/TET-MYCN mouse at P12, showing extensive retinoblastoma. The inset image is of tumor cells (original magnification, ×100) and shows Homer Wright rosettes typical of human and mouse retinoblastomas. O.N., optic nerve. (D) Kaplan-Meier curve showing the time to unilateral and then bilateral retinoblastoma formation in Rb/TET-MYCN (Rb KO MYCN) mice. (E) H&E staining of retinoblastoma-containing eye from an Rb/TET-MYCN model (inset: original magnification, ×100). Black scale bar: 500 μm; white scale bar: 25 μm.