Figure 6. In vitro activity of primary T cells transduced with individual amino acid residues at 46β.

CD8⁺ or CD4⁺ positive C57Bl/6 T cells were isolated and transduced with either 2C (left panel) or m33 (right panel) DNA encoding single amino acid residues at 46β. T cell populations were incubated with indicated stimulus for 24 hours and assayed for IFN-γ production. OVA and SIY peptides were added at 10 μM with T2-K^b cells. Error bars represent standard deviation of two separate experiments performed with each variant.