Table 2.
Primers and PCR conditions used to identify immune-deficient piglets by molecular genotype
| SNP name | Allele | Product size | Forward primer sequence | Reverse primer sequence | T, °C | Extension time, s |
|---|---|---|---|---|---|---|
| ASGA- | A | 177 | TCCTCTGACCAAGCCTCTGT | TCGTCCATGTACCAGAGCCT | 56 | 30 |
| 0048074 | C | 177 | TCCTCTGACCAAGCCTCTGT | CGTCCATGTACCAGAGCCG | 56 | 30 |
| ASGA- | A | 659 | AACCAGTCCCTGACCAACTG | TCCATATTTGTTAAGGGCAGTAATCT | 54 | 30 |
| 0048114 | G | 131 | TGCTCAGAGCTTTACATGGATTTAG | GGCCCATGTTGACATAAAGC | 54 | 30 |
| ALGA- | A | 643 | TCCTCTGCAGGGTTTCAAAG | CAGGGTGTGGGACTTTGTT | 54 | 30 |
| 0059043 | G | 127 | TCAGCTTGGGCAGCTAGG | CCACAGGCACATTGATCTTG | 54 | 30 |
| H3GA- | A | 576 | AGTTGAAATCAAAGTATCCCAA | AACTGTAACAAGCGTCCCTTTCT | 55 | 30 |
| 0030245 | C | 576 | AGTTGAAATCAAAGTATCCCAA | AACTGTAACAAGCGTCCCTTTCG | 55 | 30 |
| ALGA- | A | 593 | GGTATTCTCCTCCTCTACCTCT | CTGGATTGGCAGAGGCTCTTTAT | 55 | 30 |
| 059061 | G | 593 | GGTATTCTCCTCCTCTACCTCT | CTGGATTGGCAGAGGCTCTTTAC | 55 | 30 |
| ALGA- | A | 475 | GAATGGGAGGTGAGTAAGTAAA | CCAGCTGCAAGGGAGACT | 55 | 30 |
| 0059066 | C | 475 | GAATGGGAGGTGAGTAAGTAAA | CCAGCTGCAAGGGAGACG | 55 | 30 |
| ALGA- | A | 425 | AGCATTAAGACTGTGTGTGTGT | GGTCAAAGTCGTGGGTGTGTTT | 55 | 30 |
| 0059080 | G | 425 | AGCATTAAGACTGTGTGTGTGT | GGTCAAAGTCGTGGGTGTGTTC | 55 | 30 |
| ASGA- | ASGA- | ALGA- | H3GA- | ALGA- | ALGA- | ALGA- | |
|---|---|---|---|---|---|---|---|
| 0048074 | 0048114 | 0059043 | 0030245 | 059061 | 0059066 | 0059080 | |
| Location | 51153137 | 51812252 | 51975024 | 52066694 | 52086867 | 52109172 | 52174549 |
| hl2 | C | G | G | C | A | C | A |
| h16 | C | A | G | C | A | C | A |
| Normal | A | G | A | A | G | A | G |
The bottom section of the table shows the locations and genotypes of SNPs for chromosomes that carry normal, h12, and h16 haplotypes, as described previously [10]. T, annealing temperature.