Table 2.

Top CpG methylation sites associated^* with plasma adiponectin in GOLDN (n=991) (false discovery rate <0.2) and corresponding estimates of association from the replication analysis^** in BHS (White n=592, Black n=243). Associations with CpGs that successfully replicated in at least one BHS racial group (P<0.05/34= 0.001) are in bold.

				GOLDN		BHS White		BHS Black
Marker	Chromosome	Region	Gene	Regression Coefficient ± SE	P-value	Regression Coefficient ± SE	P-value	Regression Coefficient ± SE	P-value
cg00574958	11	North shore	CPT1A	0.01 ± 0.001	3.4x10−13	0.006 ± 0.001	0.0005	0.0006 ± 0.003	0.18
cg21212076	9	--	FAM166B	−0.02 ± 0.003	7.8x10−7	−0.002 ± 0.004	0.61	0.001 ± 0.005	0.85
cg22964469	11	--	SORL1	0.007 ± 0.001	8.8x10−7	0.0009 ± 0.002	0.56	0.004 ± 0.002	0.01
cg14266156	17	--	Intergenic	0.007 ± 0.001	1.6x10−6	−0.0004 ± 0.002	0.78	−0.002 ± 0.003	0.56
cg19828789	5	--	Intergenic	−0.008 ± 0.002	2.8x10−6	0.0003 ± 0.002	0.84	−0.0008 ± 0.002	0.66
cg05358404	20	Island	RTEL1	0.02 ± 0.003	2.9x10−6	0.006 ± 0.004	0.21	−0.0002 ± 0.005	0.96
cg05931265	11	South shore	UBASH3B	0.006 ± 0.001	3.0x10−6	−0.0004 ± 0.002	0.86	−0.006 ± 0.003	0.05
cg19393762	19	--	TNNT1	−0.009 ± 0.002	3.6x10−6	−0.002 ± 0.002	0.34	−0.0002 ± 0.003	0.95
cg07895124	16	--	GPR114	−0.008 ± 0.002	4.1x10−6	−0.0004 ± 0.002	0.81	−0.0003 ± 0.003	0.89
cg25649039	17	--	PRKCA	−0.01 ± 0.002	4.6x10−6	−0.002 ± 0.002	0.47	−0.004 ± 0.003	0.24
cg24787076	5	North shore	RPL37	0.004 ± 0.0009	4.7x10−6	0.002 ± 0.001	0.09	0.003 ± 0.002	0.09
cg19750657	13	--	UFM1	−0.008 ± 0.002	5.2x10−6	−0.01 ± 0.003	0.0002	0.003 ± 0.006	0.66
cg05879734	2	--	Intergenic	0.003 ± 0.0007	6.1x10−6	0.002 ± 0.001	0.12	0.005 ± 0.002	0.02
cg26797723	3	Island	WNT5A	−0.004 ± 0.001	6.3x10−6	0.0005 ± 0.0006	0.46	−0.003 ± 0.001	0.008
cg18810022	2	--	Intergenic	0.008 ± 0.002	5.4x10−6	0.0008 ± 0.002	0.60	−0.0002 ± 0.002	0.93
cg26985149	17	Island	Intergenic	0.005 ± 0.001	7.4x10−6	−0.002 ± 0.002	0.34	0.0002 ± 0.003	0.94
cg06710295	8	Island	MTSS1	0.002 ± 0.0005	7.8x10−6	0.0001 ± 0.001	0.89	0.0004 ± 0.001	0.74
cg16597079	6	--	TRERF1	0.006 ± 0.001	7.9x10−6	−0.0001 ± 0.001	0.92	0.002 ± 0.002	0.23
cg11552078	19	South shore	TIMM50	0.005 ± 0.001	7.9x10−6	0.001 ± 0.002	0.62	−0.006 ± 0.003	0.03
cg18758976	6	--	SYNJ2	0.007 ± 0.002	7.9x10−6	−0.001 ± 0.001	0.36	−0.0003 ± 0.002	0.88
cg18244544	19	North shore	ZNF224	0.002 ± 0.0004	8.9x10−6	0.0003 ± 0.001	0.70	0.0009 ± 0.001	0.36
cg26894079	11	--	ASAM	0.01 ± 0.002	9.0x10−6	0.006 ± 0.003	0.02	0.006 ± 0.004	0.14
cg18098839	3	--	GOLIM4	0.004 ± 0.001	9.7x10−6	0.005 ± 0.002	0.005	0.002 ± 0.003	0.44
cg00325866	15	North shore	C2CD4A	0.001 ± 0.0002	9.9x10−6	−0.0004 ± 0.0005	0.45	−0.0006 ± 0.0007	0.37
cg15183258	15	--	MGA	−0.02 ± 0.004	1.0x10−5	−0.001 ± 0.003	0.62	0.001 ± 0.004	0.77
cg14652230	3	North shore	NICN1	0.002 ± 0.0005	1.1x10−5	−0.0006 ± 0.0008	0.46	0.001 ± 0.001	0.41
cg09251317	14	--	Intergenic	0.008 ± 0.002	1.4x10−5	−0.0004 ± 0.002	0.80	0.007 ± 0.003	0.01
cg26403843	5	North shelf	RNF145	−0.008 ± 0.002	1.5x10−5	−0.02 ± 0.004	0.0004	−0.01 ± 0.006	0.04
cg08775774	12	--	CCDC62	0.002 ± 0.0004	1.5x10−5	−0.0005 ± 0.0006	0.47	−0.0002 ± 0.0008	0.83
cg17593721	5	South shore	HSD17B4	0.003 ± 0.0007	1.5x10−5	0.0004 ± 0.001	0.73	−0.003 ± 0.002	0.09
cg23923820	1	South shore	PKLR	−0.01 ± 0.002	1.5x10−5	−0.0004 ± 0.002	0.85	−0.003 ± 0.004	0.41
cg05466767	16	North shelf	FTSJD1	0.005 ± 0.001	1.6x10−5	−0.0009 ± 0.0007	0.18	−0.002 ± 0.001	0.58
cg19595760	1	South shelf	MAN1C1	0.009 ± 0.002	1.8x10−5	0.001 ± 0.002	0.63	−0.0008 ± 0.003	0.82
cg14584616	7	North shelf	HEATR2	−0.008 ± 0.002	1.8x10−5	0.0001 ± 0.001	0.91	−0.001 ± 0.002	0.58

^*Association estimates in GOLDN were obtained from linear mixed models with ln-transformed adiponectin as a predictor and methylation β score as the outcome, adjusted for age, sex, study site, T-cell purity principal components (fixed effects) and family relatedness (random effect).

^**Association estimates in BHS were obtained from linear mixed models with ln-transformed adiponectin as a predictor and methylation β score as the outcome, adjusted for age, sex, white blood cell counts (fixed effects) and batch (random effect).