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. 2017 Feb 28;23(8):1477–1488. doi: 10.3748/wjg.v23.i8.1477

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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Analysis of peripheral blood IL-12+ M1 and IL-10+ M2 monocytes and the concentrations of plasma IL-10 and IL-12. Peripheral blood mononuclear cells (PBMCs) were isolated from individual subjects and stimulated with lipopolysaccharide /phorbol myristate acetate/ionomycin in vitro. The cells were stained in duplicate with BV510-anti-CD14 and PE/Cy7-anti-CD163 or isotype controls. The cells were fixed, and permeabilized, followed by intracellular staining with BV421-anti-IL-12 and PE-CF594-anti-IL-10. The numbers of peripheral blood CD14+CD163-IL-12+ M1 and CD14+CD163+IL-10+ M2 monocytes in individual subjects were determined by flow cytometry. The concentrations of plasma IL-10 and IL-12 in individual subjects were determined by cytometric bead analysis. Data are representative FACS charts or the mean numbers of each type of cells and the mean values of each type of cytokine in individual subjects. The horizontal lines indicate the median values for each group. FSC: Forward scatter; SSC: Side scatter; HC: Healthy control; MAP: Mild acute pancreatitis.