Fig. 4.

Validation of enrichments of acetyl (685)-STAT3 following HDAC inhibition at the loci of genes with either up or downregulated expression. a, b Chromatin for ChIP was prepared from SAHA or diluent-treated BMDSCs using antibodies against acetyl (685)-STAT3 and matched IgG control. Fold enrichment at each site was determined based on normalized values in the manner of acetyl (685)-STAT3-IP samples versus IgG-IP samples. Acetyl-STAT3 bindings to a random selection of 18 STAT3 binding peaks that were associated with upregulated (a) or with downregulated genes (b) were assessed by ChIP-qPCR. Data were from 3 independent experiments (mean ± SEM). p values were obtained using an unpaired t test. * p < 0.05, ** p < 0.01. NS = Not significant. c The enrichments of STAT3 and H3K4me3 at gene loci, including Zmpste24, Uvrag and Pa2g4, were shown by IGV.