Fig. 8.

Validation of enrichments for acetyl (685)-STAT3 binding at gene loci that are related to immune regulation pathways as predicted by MSigDB. a–c Chromatin for ChIP was prepared from SAHA or diluent-treated BMDSCs using antibodies against acetyl (685)-STAT3 and matched IgG control. Fold enrichment at each site was determined based on normalized values in the manner of acetyl (685)-STAT3-IP samples versus IgG-IP samples. a Confirmation of genes targeted by acetyl-STAT3 and involved in the IL-10 signaling pathway. b Confirmation of genes targeted by acetyl (685)-STAT3 and involved in the cell cycle G1 phase. c Confirmation of genes targeted by acetyl (685)-STAT3 and involved in TNF/stress-related signaling pathway. Combined results (mean ± SEM) were from at least 3 independent experiments. p values were obtained using an unpaired t test. ** p < 0.01, * p < 0.05. NS = Not significant.