Figure 1. SMCs induces the death of glioblastoma cells in the presence of cytokines or oncolytic viruses.

(a) Alamar blue viability assay of human (M059K, SNB75, U118) and mouse (CT-2A, GL261) glioblastoma cells treated with vehicle or 5 μM LCL161 (SMC) and 0.1 ng ml⁻¹ of TNF-α or 0.01 MOI of VSVΔ51 for 48 h. Error bars, mean, s.d. n=4. (b) The indicated primary mouse NF1^−/+p53^−/+ lines were treated with vehicle or 5 μM LCL161 and 0.01% BSA, 1 ng ml⁻¹ TNF-α or the indicated MOI of a nonspreading version of VSVΔ51 (VSVΔ51ΔG) for 48 h, and viability was assessed by Alamar blue. Error bars, mean, s.d. n=4. (c) Alamar blue viability assays of human brain tumour-initiating cells (BTICs) treated with vehicle or 5 μM LCL161 and 0.001 MOI of VSVΔ51 or Maraba-MG1 for 48 h. Error bars, mean, s.d. n=3. (a,b) Representative data from three independent experiments using biological replicates. Statistical significance was compared with vehicle and BSA treatment using ANOVA using Dunnett’s multiple comparison test. Significance is reported if P<0.0001 (*).