Table 2.
Details of 15 microsatellite loci developed for Mustelus mustelus
| Locus name | Contig ID | Primer sequence | Motif | PCR product size | T A (°C) | BLAST hit | PAGE results | Accession no. |
|---|---|---|---|---|---|---|---|---|
| Mmu1 | 603691 889 5405 | F‐CCCCATTTGCAAACAGAGTT | (AT)7 | 210 | 57 | Danio rerio | Polymorphic | KX261856 |
| R‐ATTTCCCGCTGTTACATTGC | ||||||||
| Mmu2 | 17731961 828 2935 | F‐TTGTCTGCAGGAAACACAGC | (AC)6 | 163 | 56 | Cyprinus carpio | Polymorphic | KX261857 |
| R‐GCATCGTGTGAAATGGGAAT | ||||||||
| Mmu3 | 19222092 770 1754 | F‐ATACACGGACCGACTCGAAC | (TC)7 | 240 | 56 | Astyanax mexicanus | Polymorphic | KX261858 |
| R‐TAATGCCGAGATCAGGAACC | ||||||||
| Mmu4 | 26403340 740 2107 | F‐TCCATCCAGCGTTAAAGGAC | (TG)7 | 173 | 56 | Astyanax mexicanus | Polymorphic | KX261859 |
| R‐GCACCAGAGCTTCCCATTTA | ||||||||
| Mmu5 | 12961358 707 1755 | F‐ACCACTCCCTGCAGCACTAC | (CTC)6 | 282 | 57 | Callorhinchus milii | Polymorphic | KX261860 |
| R‐AGGAGATGCTTTGGCACTTG | ||||||||
| Mmu6 | 14682365 1857 7998 | F‐CACCGGAGACCTCTAACTGG | (CGC)6 | 212 | 57 | Chrysemys picta bellii | Polymorphic | KX261861 |
| R‐CGATGATGATGAAGGACGTG | ||||||||
| Mmu7 | 18961995 1089 3100 | F‐TCCCTCATTTGCTTCAGGAG | (GCT)5 | 219 | 57 | Callorhinchus milii | Polymorphic | KX261862 |
| R‐CGACATGAAACGCAGAAAGA | ||||||||
| Mmu8 | 26836431 1069 3092 | F‐AGTAAGGCGCGCTATGATTG | (CAG)5(TGT)5 | 431 | 56 | Callorhinchus milii | Polymorphic | KX261863 |
| R‐TAGAAGTCATCGCCCTCCAC | ||||||||
| Mmu9 | 7951092 402 890 | F‐ACGGTTCTGAGCAATCGTCT | (GAAT)5 | 172 | 56 | Callorhinchus milii | Monomorphic | KX261864 |
| R‐TGCGATATTCGTCAGGTGAA | ||||||||
| Mmu10 | 11748443 647 1363 | F‐AATCCTGAGCACCAGGACAC | (CATA)5 | 299 | 56 | Squalus acanthias | Monomorphic | KX261865 |
| R‐TGTGTGAATTCCCCAGATGA | ||||||||
| Mmu11 | 61216 525 1150 | F‐ATCTTGTTAACCGCCGACAG | (CAA)5 | 211 | 56 | Callorhinchus milii | Polymorphic | KX261866 |
| R‐CGCCATGTTGATCGAAGTAA | ||||||||
| Mmu12 | 1178354 | F‐GAGCAGCCAAGCATTAGTCC | (GAT)6 | 208 | 56 | Callorhinchus milii | Monomorphic | KX261867 |
| R‐CGGCTTCAGAAATTGGAATC | ||||||||
| Mmu13 | 14447036 | F‐TCATTCCTCACACCCACTCA | (GCA)5 | 112 | 56 | Squalus acanthias | Polymorphic | KX261868 |
| R‐AGATCCAGGAGCGAAGAACA | ||||||||
| Mmu14 | 12929751 492 1089 | F‐ACCGCTTGCTTCTGTTGAGT | (AGC)6 | 186 | 58 | Callorhinchus milii | Polymorphic | KX261869 |
| R‐TCGCACAGACTGATTGAAGG | ||||||||
| Mmu15 | 14824632 | F‐CACCTGATTGAGCAGGAGGT | (CTC)5 | 173 | 58 | Squalus acanthias | Monomorphic | KX261870 |
| R‐TATGGAGGTTGGGATTGCAG |
Annealing temperature in °C (T A).