Table A1.
Characteristics of two polymorphic microsatellite multiplex assays for Mustelus palumbes based on two sampling ocean basins in South Africa, Southeast Atlantic Ocean (SEAO) and Southwest Indian Ocean (SWIO)
| Locus | Microsatellite repeat motif | [P] | Dye | Ocean basin | N | Size range (bp) | N A | A R | H O | H E | PIC | F IS | P HW | Fr NULL |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mmu2 | (AC)6 | 0.2 | FAM | SEAO | 10 | 139–181 | 4 | 3.3 | 0.40 | 0.36 | 0.33 | −0.108 | 1.000 | 0.000 |
| Unknown | 15 | 3 | 2.3 | 0.14 | 0.20 | 0.19 | 0.307 | 0.111 | 0.000 | |||||
| SWIO | 12 | 9 | 6.9 | 0.67 | 0.85 | 0.79 | 0.225 | 0.002 | 0.038 | |||||
| Mmu3 | (TC)7 | 0.2 | NED | SEAO | 10 | 228–244 | 5 | 4.5 | 0.60 | 0.70 | 0.62 | 0.150 | 0.025 | 0.067 |
| Unknown | 15 | 7 | 6.1 | 0.80 | 0.86 | 0.81 | 0.072 | 0.172 | 0.022 | |||||
| SWIO | 12 | 5 | 4.4 | 0.60 | 0.76 | 0.67 | 0.217 | 0.238 | 0.074 | |||||
| Mmu4 | (TG)7 | 0.2 | VIC | SEAO | 10 | 157–201 | 2 | 2.0 | 0.30 | 0.27 | 0.22 | −0.125 | 1.000 | 0.000 |
| Unknown | 15 | 6 | 4.1 | 0.80 | 0.65 | 0.57 | −0.235 | 0.000 | 0.018 | |||||
| SWIO | 12 | 9 | 6.7 | 0.83 | 0.85 | 0.79 | 0.022 | 0.001 | 0.000 | |||||
| Mmu8 | (CAG)5(TGT)5 | 0.3 | VIC | SEAO | 10 | 410–438 | 9 | 7.6 | 0.80 | 0.87 | 0.81 | 0.089 | 0.368 | 0.031 |
| Unknown | 15 | 2 | 8.0 | 0.85 | 0.90 | 0.85 | 0.057 | 0.525 | 0.000 | |||||
| SWIO | 12 | 3 | 7.3 | 0.50 | 0.88 | 0.83 | 0.443 | 0.000 | 0.177 | |||||
| Mmu11 | (CAA)5 | 0.3 | PET | SEAO | 10 | 203–212 | 11 | 1.9 | 0.00 | 0.19 | 0.16 | 1.000 | 0.053 | 0.204 |
| Unknown | 15 | 1 | 1.0 | 0.00 | 0.00 | 0.00 | N.A. | N.A. | 0.001 | |||||
| SWIO | 12 | 7 | 4.4 | 0.10 | 0.74 | 0.65 | 0.871 | 0.000 | 0.353 | |||||
| Mmu13 | (GCA)5 | 0.2 | NED | SEAO | 10 | 85–118 | 9 | 3.0 | 0.43 | 0.67 | 0.55 | 0.379 | 0.329 | 0.135 |
| Unknown | 15 | 5 | 4.8 | 0.73 | 0.67 | 0.61 | −0.096 | 0.203 | 0.026 | |||||
| SWIO | 12 | 7 | 5.3 | 0.50 | 0.71 | 0.65 | 0.309 | 0.014 | 0.057 | |||||
| MPS1 (mean) | – | – | – | – | 5.8 | 4.7 | 0.503 | 0.619 | 0.561 | 0.210 | – | 0.067 | ||
| Mmu1 | (AT)7 | 0.2 | VIC | SEAO | 10 | 180–214 | 2 | 2.0 | 0.20 | 0.53 | 0.38 | 0.633 | 0.075 | 0.200 |
| Unknown | 15 | 6 | 4.8 | 0.73 | 0.71 | 0.65 | −0.037 | 0.133 | 0.000 | |||||
| SWIO | 12 | 6 | 5.4 | 0.50 | 0.82 | 0.75 | 0.400 | 0.005 | 0.168 | |||||
| Mmu5 | (CTC)6 | 0.3 | FAM | SEAO | 10 | 253–291 | 3 | 2.9 | 0.30 | 0.62 | 0.49 | 0.526 | 0.061 | 0.199 |
| Unknown | 15 | 6 | 4.5 | 0.54 | 0.66 | 0.60 | 0.196 | 0.159 | 0.012 | |||||
| SWIO | 12 | 7 | 5.3 | 0.58 | 0.77 | 0.70 | 0.252 | 0.001 | 0.103 | |||||
| Mmu6 | (CGC)6 | 0.3 | PET | SEAO | 10 | 203–213 | 4 | 4.0 | 0.50 | 0.76 | 0.67 | 0.357 | 0.034 | 0.124 |
| Unknown | 15 | 6 | 5.0 | 0.64 | 0.73 | 0.67 | 0.127 | 0.611 | 0.018 | |||||
| SWIO | 12 | 6 | 5.7 | 0.56 | 0.81 | 0.74 | 0.328 | 0.012 | 0.129 | |||||
| Mmu7 | (GCT)5 | 0.2 | NED | SEAO | 10 | 201–219 | 4 | 3.5 | 0.56 | 0.58 | 0.48 | 0.036 | 1.000 | 0.000 |
| Unknown | 15 | 6 | 4.7 | 0.64 | 0.62 | 0.57 | −0.045 | 0.555 | 0.000 | |||||
| SWIO | 12 | 8 | 6.5 | 0.92 | 0.85 | 0.79 | −0.080 | 0.071 | 0.000 | |||||
| Mmu14 | (AGC)6 | 0.2 | FAM | SEAO | 10 | 163–209 | 4 | 3.8 | 0.40 | 0.67 | 0.58 | 0.419 | 0.013 | 0.147 |
| Unknown | 15 | 6 | 4.7 | 0.67 | 0.74 | 0.67 | 0.097 | 0.183 | 0.028 | |||||
| SWIO | 12 | 12 | 9.2 | 0.83 | 0.93 | 0.88 | 0.109 | 0.125 | 0.044 | |||||
| MPS2 (mean) | – | – | – | – | 5.7 | 4.8 | 0.571 | 0.720 | 0.641 | 0.221 | – | 0.078 | ||
| Overall (mean) | – | – | – | – | 5.8 | 4.7 | 0.534 | 0.665 | 0.597 | 0.215 | – | 0.072 |
Primer concentration in the final reaction as μmol/L per primer ([P]); number of individuals (N); number of alleles per locus (N A); allelic richness (A R); observed heterozygosity (H O); expected heterozygosity (H E); polymorphic information content (PIC); probability of conformity to Hardy–Weinberg expectations (P HW); null allele frequency (Fr NULL). Mean values for each multiplex assay and overall are indicated in bold.