Table A2.
Characteristics of two polymorphic microsatellite multiplex assays for Galeorhinus galeus based on two sampling ocean basins in South Africa, Southeast Atlantic Ocean (SEAO) and Southwest Indian Ocean (SWIO)
| Locus | Microsatellite repeat motif | [P] | Dye | Ocean basin | N | Size range (bp) | N A | A R | H O | H E | PIC | F IS | P HW | Fr NULL |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mmu2 | (AC)6 | 0.2 | FAM | SEAO | 14 | 126–172 | 9 | 6.9 | 0.57 | 0.80 | 0.75 | 0.295 | 0.006 | 0.101 |
| SWIO | 10 | 9 | 8.4 | 0.90 | 0.91 | 0.85 | 0.012 | 0.164 | 0.000 | |||||
| Mmu3 | (TC)7 | 0.2 | NED | SEAO | 14 | 230–248 | 7 | 5.8 | 0.79 | 0.79 | 0.72 | 0.000 | 0.104 | 0.000 |
| SWIO | 10 | 5 | 5.0 | 1.00 | 0.80 | 0.72 | −0.268 | 0.064 | 0.000 | |||||
| Mmu4 | (TG)7 | 0.2 | VIC | SEAO | 14 | 162–176 | 5 | 4.6 | 0.71 | 0.74 | 0.67 | 0.037 | 0.004 | 0.000 |
| SWIO | 10 | 8 | 7.3 | 0.90 | 0.87 | 0.80 | −0.038 | 0.081 | 0.000 | |||||
| Mmu8 | (CAG)5(TGT)5 | 0.3 | VIC | SEAO | 11 | 405–436 | 10 | 8.5 | 0.82 | 0.90 | 0.84 | 0.095 | 0.007 | 0.000 |
| SWIO | 8 | 9 | 9.0 | 1.00 | 0.93 | 0.85 | −0.087 | 0.218 | 0.000 | |||||
| Mmu11 | (CAA)5 | 0.3 | PET | SEAO | 13 | 198–236 | 8 | 6.4 | 0.38 | 0.73 | 0.68 | 0.485 | 0.000 | 0.179 |
| SWIO | 10 | 11 | 9.7 | 0.60 | 0.93 | 0.87 | 0.365 | 0.002 | 0.147 | |||||
| Mmu13 | (GCA)5 | 0.2 | NED | SEAO | 14 | 89–124 | 8 | 6.7 | 0.93 | 0.81 | 0.76 | −0.154 | 0.394 | 0.000 |
| SWIO | 10 | 6 | 5.2 | 0.70 | 0.57 | 0.52 | −0.235 | 1.000 | 0.000 | |||||
| MPS1 (mean) | – | – | – | – | 7.9 | 7.0 | 0.78 | 0.81 | 0.75 | 0.042 | 0.036 | |||
| Mmu1 | (AT)7 | 0.2 | VIC | SEAO | 14 | 192–218 | 10 | 7.8 | 0.79 | 0.88 | 0.83 | 0.106 | 0.571 | 0.000 |
| SWIO | 10 | 9 | 7.8 | 0.90 | 0.84 | 0.78 | −0.073 | 0.045 | 0.000 | |||||
| Mmu5 | (CTC)6 | 0.3 | FAM | SEAO | 14 | 263–287 | 7 | 5.2 | 0.43 | 0.65 | 0.60 | 0.350 | 0.032 | 0.113 |
| SWIO | 10 | 8 | 7.7 | 0.80 | 0.89 | 0.83 | 0.111 | 0.092 | 0.036 | |||||
| Mmu6 | (CGC)6 | 0.3 | PET | SEAO | 14 | 183–220 | 10 | 8.1 | 0.71 | 0.88 | 0.83 | 0.193 | 0.022 | 0.035 |
| SWIO | 10 | 8 | 7.3 | 0.60 | 0.87 | 0.80 | 0.321 | 0.058 | 0.138 | |||||
| Mmu7 | (GCT)5 | 0.2 | NED | SEAO | 14 | 200–222 | 9 | 6.6 | 0.64 | 0.73 | 0.68 | 0.127 | 0.199 | 0.029 |
| SWIO | 10 | 10 | 8.9 | 0.90 | 0.89 | 0.83 | −0.013 | 0.088 | 0.000 | |||||
| Mmu14 | (AGC)6 | 0.2 | FAM | SEAO | 14 | 165–211 | 6 | 4.5 | 0.57 | 0.60 | 0.54 | 0.046 | 0.360 | 0.000 |
| SWIO | 10 | 7 | 6.3 | 0.70 | 0.77 | 0.70 | 0.094 | 0.234 | 0.000 | |||||
| MPS2 (mean) | – | – | – | – | 8.4 | 7.0 | 0.70 | 0.80 | 0.74 | 0.126 | 0.035 | |||
| Overall (mean) | – | – | – | – | 8.14 | 7.00 | 0.74 | 0.81 | 0.75 | 0.080 | 0.035 |
Primer concentration in the final reaction as μmol/L per primer ([P]); number of individuals (N); number of alleles per locus (N A); allelic richness (A R); observed heterozygosity (H O); expected heterozygosity (H E); polymorphic information content (PIC); probability of conformity to Hardy–Weinberg expectations (P HW); null allele frequency (Fr NULL). Mean values for each multiplex assay and overall are indicated in bold.