Figure 2. Maternal resveratrol supplementation increased BAT metabolic activity in male HFD offspring at weaning.
A, body weight (n = 8). B, fat index (percentage of fat pad weight relative to the whole body weight) of BAT, inguinal WAT and epididymal WAT (n = 8). C, representative H&E staining and UCP1 staining for BAT sections (n = 8). D, mRNA expression of thermogenic genes in BAT (n = 6). E and F, immunoblotting analysis for thermogenic genes and AMPKα and Sirt1 (n = 6). G–I, blood glucose (G) and serum triglyceride (H) and insulin concentrations (I,) of the offspring at weaning (n = 8). * P < 0.05 and ** P < 0.01. Data are shown as means + SEM. Abbreviations: AMPKα, AMP‐activated protein kinase; H&E, Haematoxylin and Eosin; Sirt1, Sirtuin 1; UCP1, uncoupling protein 1; for other abbreviations, see legend to Fig. 1. [Color figure can be viewed at wileyonlinelibrary.com]