A and B, representative H&E staining and UCP1 staining (A) and average adipocyte diameters (B) in IngWAT (n = 8). C, mRNA expression of thermogenic genes and beige adipocyte‐selective markers in IngWAT (n = 6). D, mRNA expression of PPARγ and FABP4 in IngWAT (n = 6). E and F, immunoblotting analysis of thermogenic genes and AMPKα and Sirt1 in IngWAT (n = 6). *
P < 0.05 and **
P < 0.01. Data are shown as means + SEM. Abbreviations: AMPKα, AMP‐activated protein kinase; FABP4, fatty‐acid binding protein 4; H&E, Haematoxylin and Eosin; PPARγ, peroxisomal proliferator‐activated receptor γ; Sirt1, Sirtuin 1; UCP1, uncoupling protein 1; for other abbreviations, see legend to Fig. 1. [Color figure can be viewed at wileyonlinelibrary.com]