Figure 4.

Efficiency of PNA-TAT in treatment of MRSA Sanger 252 infected C. elegans. L4-stage worms were grown on tryptic soy agar plates seeded with a lawn of bacteria for 2 hours. Worms were treated with anti-rpoA PNA-TAT at 1 × MIC, 2 × MIC or 3 × MIC for 18 hours using vancomycin and sterile water as controls. Worms were lysed, colony forming units (CFUs) were counted, and the percent bacterial reduction per worm in treated groups was calculated (relative to the untreated control groups). 10 worms (tested in triplicate) were used for each treatment. The results are presented as mean ± SD from two independent experiments. Data without error bars indicate that the SD is too small to be seen. *P ≤ 0.05 using two-tailed Student's t-test are deemed significant. PNA, peptide nucleic acid; MRSA, methicillin-resistant S. aureus.