Fig 3.

Scale Bar = 5 mm The viability of E. coli after treatment with normal human serum and in combination with oriented and non-oriented microparticles (A) The addition of 1% serum resulted in significantly lower colony formation than bacteria alone. When 1 μm 2:1 oriented microparticles were added at a 200:1 ratio with 1% serum, E. coli viability was significantly increased in comparison to treatment with serum alone. The 1 μm 2:1 non-oriented microparticles were also added at a 200:1 ratio with 1% serum and resulted in a significant increase in bacterial death. The CH50 values presented (correlating to TCC formation) are those measured on the surface of the microparticle. * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001. Relating TCC binding on microparticle surface to cytotoxicity (B) Higher levels of TCC present on the microparticle surface correlate with lower cytotoxicity. Relating TCC binding to Fc availability (C) Flow cytometry data in which an anti-Fc fluorescent secondary antibody (DyLight® 650) was used to quantify Fc density for each given particle. X and Y error bars are present in all data points.