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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Nano Lett. 2016 Aug 25;16(9):5951–5961. doi: 10.1021/acs.nanolett.6b02995

Figure 6.

Figure 6

Tpm 2.1 is a downstream target of AXL during early spreading. The ROR2 ligand binding domain is not necessary for sarcomere pinching activity regulation. (A) GFP or YFP-Tpm 2.1 transfected MDA-MB-231 cells lysates were immunoprecipitated with GFP nanotrap beads, followed immunoblotting with anti-phosphotyrosine antibody under control or R428 treated conditions. (B) Relative intensity analysis of the blot. Black represents the control group, orange for R428 treated group; (n = 3 in each case; ***p < 0.001. (C) Box-and-whisker plots of median values of Dmax distributions for nontransfected or YFP-Tpm 2.1 transfected cells under control (black) or R428 (orange) treatment on 750 nm high pillars. (D) YFP-Tpm 2.1 or YFP-Tpm 2.1-Y214F transfected MDA-MB-231 cells lysates were immunoprecipitated with GFP nanotrap beads, followed by immunoblotting with antiiphosphotyrosine antibody under control or R428 treated conditions. (E) Relative intensity analysis of the blot (black for control group; orange for R428 treated group). (F) Box-and-whisker plots of median values of Dmax distributions for YFP-Tpm 2.1 or YFP-Tpm 2.1-Y214F transfected MDA-MB-231 cells under control (black) or R428 (orange) treatment on 750 nm high pillars. (G) Box-and-whisker plots of median values of T50 distributions for ROR2 silenced cells rescued with different mutants on 750 nm high pillars (***p < 0.001; **p < 0.01). (H) Cartoon model of the structure of a typical contraction unit with the relevant molecular components.