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. 2016 Sep 19;34(1):23–31. doi: 10.1007/s10815-016-0795-0

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Expression of GTSF1 transcripts in the human female germline, preimplantation embryos, and adult tissues. a Expression of GTSF1 (primers 7 F and 9R2: Size = 152 base pairs), GAPDH, and FIGLA gene transcripts by reverse transcription RT-PCR in amplified cDNA samples derived from human ovarian follicles, oocytes, and granulosa cells. The FIGLA PCR demonstrates the presence of oocyte cDNA in the ovarian follicle samples. Lane (1) Primordial follicles (n = 28 pooled follicles), (2) pooled primordial/early primary follicles (n = 45), (3) pooled primary follicles (n = 7), and (4) pooled secondary follicles (n = 7). Lanes (5) to (8) are cDNAs generated from two dissected 5 mm antral follicles (follicles a and b). Lane (5) Denuded single human GV stage oocytes isolated from the compact cumulus-enclosed oocyte complexes of a 5 mm non-luteinised antral follicle (follicle a), lane (6) granulosa cells isolated from follicle a. Lane (7) Denuded single human GV stage oocytes isolated from the compact cumulus-enclosed oocyte complexes of a 5 mm nonluteinised antral follicle (follicle b), lane 8 granulosa cells isolated from follicle b. Lanes (9) and (10) single MII oocytes, lanes 11 to 16 cDNA from cumulus granulosa cells isolated from 5–10 mm non-luteinised antral follicles containing failed fertilization metaphase II oocytes. Lanes (17) and (18) are negative PCR controls (no template, −ve). *Only weak PCR products for FIGLA and GTSF1 were observed in one of the MII oocytes tested in this particular assay (lane 10). b Expression of GTSF1 (primers 3 F and 7R) and GAPDH in cDNAs from adult human tissue (Clontech Multiple Tissue cDNA MTC panels) and controls. Lanes (1) ovary, (2) testis, (3) spleen, (4) prostate, (5) small intestine, (6) colon, (7) thymus, (8) leukocyte, (9) pooled GV oocyte (positive control, +ve), (10) negative control (no template, −ve), and (11) mixed adult human multiple tissue sample. c GTSF1 RT-PCR (primers GTSF1 real-time F and GTSF1 real-time R), in individual human oocytes and preimplantation embryos. cDNAs were derived from pooled metaphase II oocytes (n = 6, lanes 1 to 6), morulae (n = 5, lanes 7–11), blastocysts (n = 7, lanes 12–18), and a negative control (−ve, no template). d GTSF1 RT-PCR covering the entire coding region, from exons 1–9, in cDNAs derived from pooled germinal vesicle (GV) oocytes (n = 6), metaphase II oocytes (n = 7), blastocysts (n = 7), testis and mixed adult human multiple tissue samples (MT)