Fig. 2.

Real-time PCR assessment of GTSF1 expression. Real-time PCR assessment of GTSF1 expression is expressed as a percentage of the housekeeping gene GAPDH and using primers GTSF1 real-time F and R and GAPDH real-time F and R. a GTSF1 expression in human fetal ovaries at different stages of gestation (total tested range was between 8 and 21 weeks) that were grouped into 8–11 weeks, 14–16 weeks, and 17–21 weeks of gestation, n = 4–5 samples per group. Bars indicate mean ± sem. b GTSF1 expression in human fetal testis at different stages of gestation (total tested range was between 8 and 19 weeks) that were grouped into 8–9 weeks, 14–16 weeks, and 17–19 weeks of gestation, n = 4 samples per group. c GTSF1 expression by real-time PCR in cDNAs from human ovarian follicles (follicle number per sample as described in Fig. 1a) and pooled germinal vesicle (GV) oocytes (n = 8), pooled metaphase II oocytes (n = 8), and adult gonads. Samples left to right: primordial follicles, primordial/early primary follicles, primary follicles, secondary follicles, pooled cDNA from germinal vesicle (GV) stage oocytes (n = 8), pooled metaphase II oocytes (n = 8), human ovary (Ambion), and human testis (Ambion). d GTSF1 expression by real-time PCR in cDNAs from human adult human tissue (Clontech Multiple Tissue cDNA panels, tissues as shown in Fig. 1b) and controls