Table 1.
PCR primers for conventional and real-time PCR
| Gene | Primer name | Sequence 5′ to 3′ |
|---|---|---|
| GTSF1 | Exon1F | GGAGGAAGGTGACTGTGAGG |
| Exon2F | CACTTGGATTCAGCTTCTTC | |
| Exon3F | GACCCTGAGAAGCTATTGCA | |
| Exon7F | CCCTGCGAGCAACATAGTTA | |
| Exon7R | TAACTATGTTGCTCGCAGGG | |
| Exon9R | CTGTATCAAAGGTTTATTTGGAAGC | |
| GTSF1 real-time F | ATTCAGCTTCTTCATTTCCAACA | |
| GTSF1 real-time R | CCTGATTTGATGGTTTTTGTCAT | |
| FIGLA | FIGLA F1* | GATAAAAAATCTCAACCGTGG |
| FIGLA R1* | CCCTCCTCTTCTTTCTTC | |
| GAPDH | GAPDH F§ | ACGGGAAGCTCACTGGCATGGC |
| GAPDH R§ | TCTTACTCCTTGGAGGCCATGTAGG | |
| GAPDH real-time F | TTGTCAAGCTCATTTCCTGGTAT | |
| GAPDH real-time R | TCTCTCTTCCTCTTGTGCTCTTG |