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. 2017 Mar 1;8:257. doi: 10.3389/fmicb.2017.00257

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Copyright © 2017 Eshaghi, Duvvuri, Isabel, Banh, Li, Peci, Patel and Gubbay.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree of EV-D68 detected globally and from Ontario, Canada. The phylogenetic tree based on the alignment of partial VP1 sequences (339 bp) of EV-D68 was inferred using the Neighbor-Joining method, evolutionary distances were calculated with the Maximum Composite Likelihood method (MCL), and 1,000 Bootstrap replicates were computed to estimate the accuracy of the phylogenetic inference using MEGA v6.06 (http://www.megasoftware.net). Ontario sequences from this study are identified with red filled circles. Sequences collected in 2014 from the USA, Italy, and Netherlands are marked with a blue square, green diamond, and light blue triangle, respectively. AFP sequences collected in 2013 obtained from GenBank are labeled with a green circle. Bootstrap values > 60% are shown next to the nodes. Branch lengths are drawn to the indicated scale, proportion of nucleotide substitutions per site. Some sub-clades are collapsed and labeled in bold to assist with visualization of other clades/sub-clades.