Skip to main content
. 2017 Mar 1;8:304. doi: 10.3389/fmicb.2017.00304

FIGURE 2.

FIGURE 2

miR-21a-5p directly regulates Caskin1 expression by targeting the 3′-UTR of Caskin1. (A) Caskin1 mRNA expression in the N2a cells after infecting with PHEV by RT-PCR. (B) The expression of Caskin1 in the N2a cells after infecting with PHEV by Western blot. (C) Caskin1 mRNA expression in the BALB/c mice brain tissue after infecting with PHEV by RT-PCR. (D) The expression of Caskin1 in the BALB/c mice brain tissue after infecting with PHEV by Western blot. (E,F) The expression of Caskin1 after transfection with the miR-21a-5p mimics or inhibitor by using RT-PCR (E) or Western blot (F). (G) The Dual-luciferase reporter construct containing the wild type or mutant 3′-UTR of Caskin1. Caskin1-WT-UTR, sequence of the putative miR-21 binding site; Caskin1-MUT-UTR, sequence of the mutant miR-21 binding site. (H) Dual-luciferase reporter activity in Hela cells 48 h post-transfection. All of the data are representative of at least three independent experiments. ∗P < 0.05, ∗∗P < 0.01 vs. normal controls.