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. 2017 Mar 1;8:304. doi: 10.3389/fmicb.2017.00304

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Copyright © 2017 Lv, Zhao, Lan, Li, Ding, Su, Lu, Song, Gao and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-21a-5p promotes PHEV replication by targeting Caskin1 in N2a cells. (A) Caskin1 mRNA levels in N2a cells 48 h post-transfection with siRNAs. (B) Caskin1 protein levels in N2a cells 48 h post-transfection with Caskin1 siRNA. (C) The levels of the viral genome RNA after transfecting with Caskin1 siRNA. (D) Western blotting was performed to examine the expression of PHEV protein. (E) IFA was performed to examine the expression of PHEV. (F) Viral titers from N2a cells transfected with Caskin1 siRNA. All of the data are representative of at least three independent experiments. ^∗P < 0.05, ^∗∗P < 0.01 vs. normal controls.