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. 2017 Mar 1;8:203. doi: 10.3389/fimmu.2017.00203

Figure 1.

Figure 1

Cathepsin E (Ctse) expression is increased in target organs during graft-versus-host disease (GVHD). (A,B) Ctse mRNA expression assessed by qPCR in colon and liver during acute GVHD [aGVHD, allogeneic stem cell transplantation; allogeneic hematopoietic stem cell transplantation (allo-SCT)], and syngeneic transplanted control animals (no GVHD, syn-SCT) at day +15 after allo-SCT in the LP/J→C57BL/6 model. Ctse expression is shown relative to the no GVHD control group. n = 5 per group (C,D) Ctse protein expression is elevated in immune cell infiltrates in the colon during acute GVHD (aGVHD) compared to syn-SCT recipients without GVHD (no GVHD). Ctse expression is shown in green, CD11c is shown in red, nuclear counterstain with DAPI is shown in blue, Ctse-expressing cells are highlighted with white arrows, same arrow positions are shown in the CD11c staining, illustrating that Ctse-positive cells are also CD11c+. Dotted line marks the border of the lamina propria to the lamina muscularis mucosae. Fluorescence images were taken using a Motic BA410 microscope with a Moticam Pro 285B and the Motic Images Plus 2.0 software. The used objective was a Plan Fluar 40×/0.75. Error bars indicate mean ± SEM, p-values in (A,B) were calculated using a double-sided Student’s t-test. Bar = 50 μm.