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. 2017 Mar 1;7:61. doi: 10.3389/fcimb.2017.00061

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Copyright © 2017 Fish, Riley, Singh, Riesbeck and Martinez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of loop 3 and 4 mutant Adr1 serum resistance when expressed on the surface of E. coli and exposed to NHS. (A,D) A single lysine to alanine substitution (AF2 and AF7). (B,E) A double lysine to alanine substitution at positions 1 and 2 (AF13 and AF17). (C,F) A triple amino acid substitution at positions 1, 2, and 3 (AF14 and AF24). Data are representative of at least 3 replicates. A one-way ANOVA with a Newman-Keuls post hoc test was performed. ^*Represent a p ≤ 0.05 and is considered significant. ns represents no significance.