Figure 3.

Restored lysozyme resistance of genetically complemented mutants. The ΔbamB and ΔfabY mutants were transformed with pJN105::bamB or pJN105::fabY plasmid, respectively. As a control, each mutant was also transformed with the empty plasmid, pJN105. Bacterial strains were grown in the presence of 1 mg/mL lysozyme for 16 h. To induce the gene expression, 0.01% L-arabinose was also added in each media. Aliquots of each culture were diluted and plated for CFU quantification. Bacterial cultures were performed in triplicate, and the mean ± SD values are displayed. ^*P < 0.05 vs. CFU of each mutant harboring pJN105.