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. 2017 Mar 1;7:59. doi: 10.3389/fcimb.2017.00059

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Copyright © 2017 Lee, Lee, Go, Park, Shin, Choi, Kim and Yoon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial growth in airway mucus secretion (AMS) prepared from primary culture of human tracheal tissues. The wild type PAO1 and its two mutants, ΔbamB and ΔfabY, were grown in three separate AMS preparations for 16 h. Aliquots of each culture were diluted and plated for CFU quantification. The values for CFU _in16h / CFU _inoculum were calculated and plotted as the growth index. Bacterial cultures were performed in triplicate, and the mean ± SD values are displayed. ^*P < 0.05 vs. CFU of PAO1 cells.