Figure 6.

Bacterial membrane integrity tests of two lysozyme-sensitive mutants. (A) Bacterial cells resuspended in distilled water were monitored for their changes in OD₆₀₀ values at 10 min intervals for 1 hr. Experiments were performed in triplicate, and the mean ± SD values are displayed. ^*P < 0.05 vs. OD₆₀₀ of PAO1 at each time point. (B) Each bacterial culture was stained with Syto9 and propidium iodide and visualized by confocal laser scanning microscope (CLSM) as described in Section Materials and Methods.