Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Relevant characteristics | References or source |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Standard lab strain | Yoon et al., 2006 |
| ΔPA0420 | PA01, PA0420 gene deleted | This study |
| ΔPA3800 | PAO1, PA3800 gene deleted | This study |
| ΔPA5174 | PAO1, PA5174 gene deleted | This study |
| ΔPA3800/pJN105 | ΔPA3800 harboring pJN105 | This study |
| ΔPA5174/pJN105 | ΔPA5174 harboring pJN105 | This study |
| ΔPA3800/pJN105::PA3800 | ΔPA3800 harboring pJN105::PA3800 | This study |
| ΔPA5174/pJN105::PA5174 | ΔPA5174 harboring pJN105::PA5174 | This study |
| E. coli strains | ||
| SM10/λpir | Kmrthi-1 thr leu tonA lacY supE recA::RP4–2-Tc::Mu pir+, for conjugal transfer | Simon et al., 1983 |
| OTHER BACTERIAL SPECIES | ||
| Staphylococcus aureus | ATCC 29213 | Lab collection |
| Bacillus subtilis | ATCC 6633 | Lab collection |
| Escherichia coli | ATCC 25922 | Lab collection |
| Salmonella typhimurium | LT2 strain | Lab collection |
| Listeria monocytogenes | ATCC 19111 | Lab collection |
| PLASMIDS | ||
| pBTK30 | Transposon vector for construction of a random mutant library, Gmr | Kim et al., 2012 |
| pCVD442 | sacB suicide vector derived from plasmid pUM24 | Lee et al., 2012 |
| pJN105 | araC-PBAD cassette cloned in pBBR1MCS-5, Gmr | Newman and Fuqua, 1999 |
| pJN105::PA3800 | pJN105 with wild-type copy of PA3800 gene under arabinose-inducible promoter | This study |
| pJN105::PA5174 | pJN105 with wild-type copy of PA5174 gene under arabinose-inducible promoter | This study |