Table 2.
Primers used in this study.
| Gene name | Direction | Primer sequence (5′−3′)a | Restriction enzymes |
|---|---|---|---|
| MUTANT CONSTRUCTION | |||
| PA0420 left | Forward | ACCTTGAGCTCGCCGAGAGATCGTCCAGGGT | SacI |
| PA0420 left | Reverse | TCCGGGTGGAAGTCGCTGACAAGGCCCATGGGCTGTCTCC | |
| PA0420 right | Forward | GGAGACAGCCCATGGGCCTTGTCAGCGACTTCCACCCGGA | |
| PA0420 right | Reverse | TAGAGGAGCTCAGGGCCAGAGGCGGATGGAT | SacI |
| PA3800 left | Forward | ACCTTGCATGCTGGTGGCAGCGCAACGGCAA | SphI |
| PA3800 left | Reverse | ACGAGCTTGCCACCGTTGCCCACCATCTCAGGCCTCTCCC | |
| PA3800 right | Forward | GGGAGAGGCCTGAGATGGTGGGCAACGGTGGCAAGCTCGT | |
| PA3800 right | Reverse | TAGAGGCATGCGATCCCCAGGGCTTCCTGCA | SphI |
| PA5174 left | Forward | ACCTTGAGCTCGTCCAGGCCGCCATCGAGTT | SacI |
| PA5174 left | Reverse | GGCGCTCAGTCGAGCATGTCGCCTGCTGCGTTGTAGCCAC | |
| PA5174 right | Forward | GTGGCTACAACGCAGCAGGCGACATGCTCGACTGAGCGCC | |
| PA5174 right | Reverse | TAGAGGAGCTCCCGGTAGCGAGGAGTTCACC | SacI |
| COMPLEMENTATION | |||
| PA3800 complementation (cloning to pJN105) | Forward | AATTCGAATTCAAGGAGATATACATATGGTGCAATGGAAACACGC | EcoRI |
| Reverse | ATATCTCTAGACTAGCGGATGGTGTAGGCGA | XbaI | |
| PA5174 complementation (cloning to pJN105) | Forward | AATTCGAATTCAAGGAGATATACATATGTCTCGACTACCGGTCATT | EcoRI |
| Reverse | ATATCTCTAGATCAGTCGAGCATGTCGCTGA | XbaI | |
a
Restriction enzyme recognition sequences are underlined.