Figure 2.

Effect of Dex on ${\text{NH}}_4^{+}$ influx in mouse isolated SMG and HSG cells. ${\text{NH}}_4^{+}$ influx was assessed by measuring changes in pH_i in SMG acini with and without 100 ng/ml Dex. The rate of ${\text{NH}}_4^{+}$ influx (gray dotted line) was determined from the pH_i recovery rate in the second phase after 20 mM NH₄Cl pulse. Bars represent the mean ± SEM (n = 5). (A) Traces of the NH₄Cl pulses used to measure ${\text{NH}}_4^{+}$ influx with 100 ng/ml Dex or 10 μM YOH in isolated SMG acini. (B) Analysis of ${\text{NH}}_4^{+}$ influx in isolated SMG acini. Bars represent the mean ± SEM (n = 4, ^*p < 0.01). Analysis of ${\text{NH}}_4^{+}$ influx in HSG cells (C,D) and SMG acini (E) in the presence of the indicated components including 100 μM Bumetanide (Bumeta). Bars represent the mean ± SEM (n = 4, ^*p < 0.01).